2005年6月9日的Nature杂志刊发了基于流式荧光技术（液态芯片）的研究论文
来自麻省理工学院、哈佛大学等国际顶级研究机构的科学家运用流式荧光技术（液态芯片），对人类已知的217 个非编码小片段mRNA 与肿瘤的关系进行了研究，研究结果刊登在2005 年6 月9 日出版的Nature 杂志上。
Nature 435, 834-838 (9 June 2005) | doi: 10.1038/nature03702
MicroRNA expression profiles classify human cancers
Jun Lu1,4,7, Gad Getz1,7, Eric A. Miska2,7,8, Ezequiel Alvarez-Saavedra2, Justin Lamb1, David Peck1, Alejandro Sweet-Cordero3,4, Benjamin L. Ebert1,4, Raymond H. Mak1,4, Adolfo A. Ferrando4, James R. Downing5, Tyler Jacks2,3, H. Robert Horvitz2,6 and Todd R. Golub1,4,6
Recent work has revealed the existence of a class of small non-coding RNA species, known as microRNAs (miRNAs), which have critical functions across various biological processes1, 2. Here we use a new, bead-based flow cytometric miRNA expression profiling method to present a systematic expression analysis of 217 mammalian miRNAs from 334 samples, including multiple human cancers. The miRNA profiles are surprisingly informative, reflecting the developmental lineage and differentiation state of the tumours. We observe a general downregulation of miRNAs in tumours compared with normal tissues. Furthermore, we were able to successfully classify poorly differentiated tumours using miRNA expression profiles, whereas messenger RNA profiles were highly inaccurate when applied to the same samples. These findings highlight the potential of miRNA profiling in cancer diagnosis.
1.Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02141, USA
2.Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, 3.and MIT Center for Cancer Research, Cambridge, Massachusetts 02139, USA
4.Department of Pediatric Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA
5.Department of Pathology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
6.Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
7.*These authors contributed equally to this work
8.†Present address: Wellcome Trust/Cancer Research UK, Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, UK
Correspondence to: Todd R. Golub1,4,6 Correspondence and requests for materials should be addressed to T.R.G. (Email:
golub@broad.mit.edu).
Information miRNA expression data have been submitted to the Gene Expression Omnibus under the series accession number GSE2564.
Received 2 February 2005; Accepted 5 May 2005
